The clinical significance of IgE-antibodies to Malassezia in children with atopic dermatitis

Background. The objective was to study the species diversity and the proportion of lipophilic yeasts of the genus Malassezia on the skin of children with atopic dermatitis and control groups, to evaluate age-related features and clinical significance of sensitization to Malassezia and IgE-antibodies to Mn-SOD in children with atopic dermatitis. Materials and methods. The study involved 144 children aged between 1 -14 years: 93 patients with atopic dermatitis, a control group of K1 - 24 children with asthma and /or AR without atopic dermatitis and K2 - 27 healthy children without skin diseases. The yeast Malassezia spp. identified by morphological and physiological characteristics and analysis of the nucleotide sequences of the D1/D2 region of 26S (LSU) rDNA. For detection of IgE antibodies to various allergens, including Malassezia, used the device and reagents ImmunoCap (Thermofisher, Sweden); IgE-antibodies to Mn-SOD was detected by the method of ISAC ImmunoCap (Thermofisher, Sweden). Results. Identified 5 species of Malassezia, absolutely dominated M. sympodialis. IgE-antibodies to Malassezia spp. were detected in 45% (42/93) of patients with atopic dermatitis: 11% - children with ADL, 22% from the ADB and 67% from the ADT. Statistically significant correlation between the level of IgE-antibodies to the yeast Malassezia spp. and severity of atopic dermatitis (SCORAD) - r=0,5928, p