2007•Progress in Biomedical Optics and Imaging - Proceedings of SPIERequires access

Elastic light scattering by cells: From Mie scattering to fractal scattering

Min Xu, Tao Wu, Jianan Y. Qu

Abstract

A unified theory for light scattering by biological cells is presented. It is shown that Mie scattering from the bare cell and the nucleus dominates cell light scattering in the forward directions. The random fluctuation of the background refractive index within the cell, behaving as a fractal random continuous medium, dominates light scattering by cells in other angles. The theory is validated by experimental angular light scattering spectra of epithelial cells for scattering angles from 1.25 to 173.8 degrees and in the spectral range from 400nm to 700nm.

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Key concepts: Scattering, Light scattering, Mie scattering, Scattering theory, Optics, Physics, Scattering length, Static light scattering