Methods for the determination of vitamin a by means of high performance liquid chromatography (HPLC)
Helmut Rückemann
Abstract
Helmut Rückemann
Abstract
A method is described for the rapid determination of Vitamin A in feeds. After saponification of the sample the vitamin is extracted with petroleum benzene. In this extract the vitamin A is determined spectrophotometrically by HPLC without any further clean-up.
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A method is described for the rapid determination of Vitamin A in feeds. After saponification of the sample the vitamin is extracted with petroleum benzene. In this extract the vitamin A is determined spectrophotometrically by HPLC without any further clean-up.
Key concepts: Saponification, High-performance liquid chromatography, Chromatography, Vitamin, Chemistry, Biochemistry