CHROMIUM DETERMINATION IN JATROPHA PLANT SAMPLES BY ASABT USING UV-VIS SPECTROPHOTOMETRIC METHOD

A very simple, selective and sensitive method is developed for the spectrophotometric determination of Cr (III). The reagent 5-Acetylsaliylamide-2-iminothiophenol (ASABT) provides a yellow colored solution with Chromium (III) in an aqueous medium. The maximum absorption of the reagent λmax shows at 350 nm. The maximum absorption of the pH range was observed at 3.0-5.0. The Beer’s law validity was 0.05-3.46 µg/ml. The Molar absorption factor i.e., 5.78 X103 L mol-1cm-1 and Sandell’s sensitivity 0.00173 µg/ml. The Specific absorption factor was 0.00916 ml g-1cm-1. The composition of metal and ligand (M: L) obtained in Job’s and Molar quantitative relation technique, are in 1:1 (M: L) ratio and 1.002 X 10-3 was calculated as the stability constant of the complex. For full-colour improvement, a ten molar chemical reagent in excess was necessary. Chromium (III) of concentration i.e., 2.12 µg/ml was used in the calculation of standard deviation for a set of ten various determinations was calculated as 0.0013 µg/ml. The calculated Relative standard deviation of the system was calculated as 0.5849% and Mean absorbance was found to be 0.220 ± 0.01. The results obtained are in excellent agreements with the nominal values.