PHYTOCHEMICAL ANALYSIS OF USEFUL PARTS OF BARBARI (OCIMUM BASILICUM LINN.) AND ITS EVALUATION FOR ANTI-MICROBIAL ACTIVITY

In recent decades, the interest in evaluating therapeutic effects of plants has increased d. Phyto-medicines have been shown to be good alternatives to synthetic chemical antimicrobial agents because of the serious side effects, antimicrobial resistance and the emergence of previously uncommon infections due to inappropriate or widespread overuse of antimicrobials. Barbari is a commonly available drug and have also been used as a folk remedy to treat various ailments. Hence Phytochemical Analysis of useful parts of Barbari (Ocimum basilicum Linn.) and its evaluation for anti-microbial activity were taken for study. The objectives of the study are Pharmacognostic study of useful parts in Barbari, Phytochemical analysis of useful parts in Barbari, Qualitative analysis of Phyto-elements present in the drug and Anti-microbial activity of Barbari on certain strains of microbes. Extraction of Arka from both leaves & seeds were done as per the norms mentioned in classics. Phytochemical Analysis of different extracts is done. Determinations of volatile oil percentage and chemical finger printing by GC-MS and HPLC of both the Arka have been conducted, along with their antimicrobial activities evaluated. The Results obtained are negative for the presence of Euginol by GC-MS & HPLC. Both the samples were found to be inactive against tested bacterial and fungal strains.