2021•PubMedOpen access

Protective effects of quercetin against hyperglycemia-induced oxidative stress in hepatic HepG2 cell line.

Amir Yarahmadi, Mostafa Moradi Sarabi, Ahmad Sayahi, Fatemeh Zal

Abstract

OBJECTIVE: model. MATERIALS AND METHODS: HepG2 cells were cultured with different concentrations of D-glucose (5.5, 30 and 50 mM) and/or 25 μM quercetin for 48 and 72 hr, respectively. The effect of treatments on cellular integrity, antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) activity, andcellular levels of glutathione (GSH) and malondialdehyde (MDA) wasdetermined. RESULTS: D-glucose had various effects on intracellular antioxidant defense atdifferent doses and time-points and quercetin could attenuate oxidative stress and modulate antioxidant defenses. CONCLUSION: The results of this study indicated that flavonoid quercetin could be proposed as an agent protecting hepatic HepG2 cells against oxidative stress associated with hyperglycemia.

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Key concepts: Quercetin, Oxidative stress, Antioxidant, Superoxide dismutase, Glutathione peroxidase, Glutathione, Glutathione reductase, Malondialdehyde