Direct organogenesis of patchouli [Pogostemon cablin Benth]

Abstract Patchouli [Pogostemon cablin Benth] is a plant that produces essential oils that are used for various industrial purposes. To increase the productivity of patchouli, quality seeds are needed. Patchouli propagation can be performed by in vitro culture such as direct or indirect organogenesis. The purpose of this study was to obtain the types of explants and composition of the media to inducing shoot through direct organogenesis. The research used a factorial completely randomized design. The first factor was the type of explants [leaves and nodus], the second factor was the concentration of BA [0.3, 0.4, and 0.5 ppm]. Data were analyzed using the F test and Duncan as posthoc test using the STAR [Statistical Tool for Agricultural Research]. The results showed shoot induction time varies from 9.3-21.7 days after culture. BA with 0.3 ppm gives the best performance in a number of the shoot and shoots height about 16.3 shoots and 2.1 cm, respectively.