2009•Zhongguo yufang shouyi xuebaoRequires access
Production and characterization of the monoclonal antibodies against NS1 protein of Japanese encephalitis virus
Bin Wang, Rong-Hong Hua, Zhao FuRong, Tianqiang Liu, Yu Hai, Juan Du, Tong Guang-zhi
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Abstract
Four monoclonal antibodies(McAb) against Japanese encephalitis virus(JEV) nonstructural protein(NS1) of SA14-14-2 strain were produced.These McAbs were generated by fusing BALB/c mice myeloma cell line SP2/0 with spleen lymphocytes from BALB/c mice immunized with prokaryotic expressed NS and were designated as 1H6,2C3,3A7 and 4C8,respectively.The McAb 1H6 belonged to the subtype of IgG2b,while 2C3,3A7 and 4C8 were identified as IgG1 subtype,and the light chains of all antibodies were κ type.The antibody titer of ascites were 1 :204 800,1:25 600,1:204 800 and 1:102 400 respectively.Western blot showed that the antibodies were specific to JEV NS1.Indirect immunofluorescence assay showed that 3 of 4 McAbs recognized JEV NS1 protein in JEV infected BHK-21 cells.McAbs reported here may provide valuable tools for further investigation of the structure and function of JEV NS1 protein.