2011•Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna)Requires access
A system biology approach to ionizing radiation response by mammalian cell lines
Angela Virelli, Entelë Gavoçi, Gastone Castellani, Isabella Zironi, E. Ceccolini, Federico Rocchi, M. Sumini, E. Belligotti
Open publisher page 3 citations
Abstract
A key issue when analyzing the biological effects of ionizing radiation is the selection of the biological endpoints. In this study we evaluated the effects of X-rays and protons on membrane ionic currents, cell growth and stress response. The membrane ionic currents amplitude showed a significant nonlinear effects. Growth curves showed a significant dose dependent decrease in the proliferation rate. The fluorescence data suggest a trend in which superoxide intracellular concentration decreases at lower irradiation doses compared to control and higher doses. These results are consistent with a signal transduction pathway where the concentration of superoxide is controlled by type and dose of ionizing radiation