Inhibitory effects of paclitaxel liposome on ovarian cancer cell

Objective To ananlyze the inhibitory effects of paclitaxel liposome to ovarian cancer cell(SKOV-3),to observe the time-concentration effect of this drug and to explore the mechanism of paclitaxel liposome effecting on the tumor cell. Methods SKOV-3 cells were cultured and divided into groups,which was added with different concentrations of paclitaxel liposome.The cells survival rate was observed by MTT,the inhibitory rate and the cell cycle by flow cytometry,and the expressions of Bcl-2 and Bax proteins by Western blot.SPSS 13.0 was used to analyze the data. Results The paclitaxel liposome could inhibit SKOV-3 cells,which showed time-concentration-dependent effect.The paclitaxel and paclitaxel liposome could both block SKOV-3 cells at G2/M phase,and cell apoptosis was more obviously along with increasing drug concentration.The Western blot results demonstrated that after using paclitaxel liposome to deal with the SKOV-3 cells,the expression of Bcl-2 protein obviously was decreased,while the expression of Bax protein increased and the value of Bax/Bal-2 up-regulated. Conclusions Paclitaxel liposome has inhibitory action and time-concentration-dependent effect on breast cancer cell line SKOV-3 as same as paclitaxel.Therefore,paclitaxel liposome has a good future for breast cancer patients.