The effect of cerbenicillin sodium on the callus induction and growth in cotton (Gossypium hirsutum)

Cerbencillin sodium promotes the formation and proliferaiton of callus from cotton (Gyssypium hirsutum) in a certain concentration range. The effect was in a curve to the concentration. The optimum concentration is about 250-1 000mg/L for the hypocotyl callus formation of Coker 201, and 250-750 mg/L for the cotyledon. cerbencillin sodium inhibited the pfoliferation of cotton callus of Coker 201 at beginning and promote in the later time. With the increase of cerbencillin sodium concentration, the inhibiting time was longer. The formation and proliferation of callus were inhibited and killed when cerbencillin sodium (concentration of 500 mg/L) and kannamycin (concentration of 70 mg/L) added at the same time.