Detection of PD4,CD44,PCNA protein and its clinical significance in human laryngeal carcinoma

Objective:To study the expression of PD4、CD 44 and PCNA proteins in laryngeal carcinoma and their relationships with the patho genesis, development and prognosis of laryngeal carcinoma.Method:Immunohistochemistry was used to study 140 cases of laryngeal carcinoma tis sues,25 cases of precarcinoma tissues,36 cases of vocal cord polyps and 13 cases of normal tissues adjacent to laryngeal carcinoma.Result:① The positive rates of PD4,CD44 and PCNA were 45.71%(64/140),64.29%(90/140) and 77.86%(109/140) in laryngeal carcinoma,which were much higher than in non-carc inoma tissues(P0.01).②The third and fourth stages laryngeal carcinoma exp ress stronger PD4 and CD44 than those of the first and second stages.Laryngeal carcinoma with cervical metastasis had higher expression than those without cerv ical metastasis.To 3 and 5 years' survival,PD4、CD44 and PCNA positive cases ha d lower chance than those negative cases(P0.01 or 0.05).③The over all pos itive rate of PD4、CD44 and PCNA was 27.86%(39/140) in laryngeal carcinoma tissu es and 5.41%(4/74) in non-carcinoma tissues.Conclusion:The high expression of PD4,CD44 and PCNA proteins maybe closely related to the path ogenesis, development and prognosis of laryngeal carcinoma.