Inhibitory effect of oxaliplatin in combination with hyperthermia on angiogenesis

Objective To study the effect of oxaliplatin in combination with hyperthermia on angiogenesis in vitro and in vivo. Methods MTT method was used to observe the influence of oxaliplatin on the proliferation of human umbilical vein endothelial cells (HUVEC) or human colon cancer cells (LOVO). The influence of oxaliplatin on HUVEC migration was evaluated by Transwell. Chick embryo chorioallantoic membrane (CAM) model was used to check whether the neovascularization of CAM could be suppressed in vivo. Results The survival rate of HUVEC was 80. 1%～42. 5% within a range of 0. 5～16μg/ml and was negatively correlated with the concentration (correlation coefficient was - 0. 943,P = 0. 005). The survival rate of LOVO cells within those doses was more than that of HUVEC. There was a synergistic anti-angiogenic effect when a combination of oxaliplatin (0. 5μg/ml, 1μg/ml and 16μg/ml) with hyperthermia was used while additional effect was shown by the combinatioin of oxaliplatin (2μg/ml, 4μg/ml and 8μg/ml) and hyperthermia in vitro. Oxaliplatin inhibited migration of HUVEC in vitro at low doses (0.25～2μg/ml), and also suppressed angiogenesis of CAM in vivo at doses of 1～4μg/ml. Conclusion The results of this experiment showed that low dose of oxaliplatin has anti-angiogenic effect in vitro, while in combination with hyperthermia has additional effect both in vivo and in vitro.