CLONING AND SEQUENCE DETERMINATION OF P22 GENE FRAGMENT OF TOXOPLASMA GONDII

Cloning and sequence determination of P22 gene fragment of Toxoplasma gondii gene fragment was amplified by PCR.After purification, the gene fragment was ligated with plasmid p5. 6 at polylinker. The recombinant plasmid p5.6/P22 was transformed into E. coli DH 5a. Positive clones were screened and indentified by PCR techniche and digestion with restriction enzyme.The sequence of inserted P22 gene fragment was also determined.Results and Conclusion Therecombinant plasmid of P22 gene fragment of Toxoplasma gomdii was successfully constructed.That would make it convenient for P22 diagnostic antigen expression.