Inhibitory Effect and Molecular Mechanism of Silencing S100A4 Gene on the Growth of Transplanted Tumor of Human Esophageal Carcinoma EC-1 Cells in Nude Mice

Objective To study the effect of S100A4siRNA on tumor growth of xenografted human esophageal squamous cell carcinoma(ESCC) cell line EC-1 in nude mice and explore its related molecular mechanism.Methods The xenografted tumor model was established in nude mice,and S100A4siRNA chemically synthesized was used to transfect the xenografted nude mice.The tumor growth was observed.The mRNA and protein expressions of S100A4,MMP-2 and E-cadherin in tumor after transfection with S100A4siRNA were detected by RT-PCR and immunohistochemistry.Results The tumor volume of S100A4siRNA transfection group was lower than that of nonsense siRNA transfection group and blank control group(P0.05),and tumor inhibitive ratio of S100A4siRNA group was higher than that of nonsense siRNA transfection group(P0.05).Furthermore,the mRNA and protein expressions of S100A4,MMP-2 in S100A4siRNA group were lower than those in nonsense siRNA group and control group(P0.05),the mRNA and protein expressions of E-cadherin in S100A4siRNA group were higher than those in nonsense siRNA group and control group(P0.05).Conclusion S100A4siRNA could effectively lead to growth inhibition of xenografted human esophageal tumor in nude mice,with down regulation of MMP-2 and up regulation of E-cadherin,which provides theoretical basis for molecular therapy of ESCC.