The influence of lanthanum chloride on the TNFα expression of murine peritoneal macrophages stimulated by lipopolysaccharide

Objective To explore the influence o f lanthanum chloride on the TNFα expression of murine peritoneal macrophages stim ulated by lipopolysaccharide (LPS). Methods Murine peritoneal macrophages (M) were isolated , cult ured and then stimulated by LPS. The influence of lanthanum chloride on the TNF α secretion and TNFαmRNA expression of murine M stimulated by LPS was determ ined by ELISA method and SYBR green fluorescence quantitative RT PCR. Forty BAL B/C mice were randomly divided into two groups and were treated by lethal dose o f LPS and lanthanum chloride processed LPS, respectively. The mortality within 7 days was observed. Results The TNFα secretion and TNFαmRNA expression level of th e M from mice treated by lanthanum chloride processed LPS were obviously lower than those by LPS only (P0.01). The mortality of the mice treated by letha l dose of LPS which has been processed by lanthanum chloride was significantly l ower than that by lethal dose of LPS only. Conclusion Lanthanum chloride possesed the capacity of lowering down the toxicity of LPS and inhibiting the TNFα secretion and TNFαmRNA expres sion in murine M stimulated by LPS .