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2006•Chung-Hua Wai K'o Tsa ChihRequires access

A pilot study on the impact of the drug resistance on the radioresistance La human pancreatic cancer cell lines

WU Wei-we

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Abstract

Objective To investigate the impact of the drug resistance on the radioresistance in human pancreatic cancer cell lines. Methods Three drug resistant pancreatic caner cell sublines induced by fluorouracil (5-FU), adriamycin (ADM) and gemcitabine respectively, SW1990/FU, SW1990/ADM and SW1990/Gz, were tested for the cell cycle and radio-sensitivity with flow cytometry and clonogenic assay. Results Compared with SW1990, the cell cycle assay indicated higher G0/G1 period percentage in SW1990/FU and SW1990/Gz, but the G2/M period percentage decreased; SW1990/FU had the same while SW1990/Gz had lower S period percentage. SW1990/ADM almost had a similar cell cycle with SW1990. Clonogenic assay showed both SW1990/FU and SW1990/Gz had greater survival fraction (SF2) than SW1990, but SW1990/ADM had seemingly similar SF2 as SW1990. Conclusion Drug resistant pancreatic cancer cell lines have reduced G2/M period percentage and increased radioresistance.

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What this paper is about

Objective To investigate the impact of the drug resistance on the radioresistance in human pancreatic cancer cell lines. Methods Three drug resistant pancreatic caner cell sublines induced by fluorouracil (5-FU), adriamycin (ADM) and gemcitabine respectively, SW1990/FU, SW1990/ADM and SW1990/Gz, were tested for the cell cycle and radio-sensitivity with flow cytometry and clonogenic assay. Results Compared with SW1990, the cell cycle assay indicated higher G0/G1 period percentage in SW1990/FU and SW1990/Gz, but the G2/M period percentage decreased; SW1990/FU had the same while SW1990/Gz had lower S period percentage. SW1990/ADM almost had a similar cell cycle with SW1990. Clonogenic assay showed both SW1990/FU and SW1990/Gz had greater survival fraction (SF2) than SW1990, but SW1990/ADM had seemingly similar SF2 as SW1990. Conclusion Drug resistant pancreatic cancer cell lines have reduced G2/M period percentage and increased radioresistance.

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Available abstract

Objective To investigate the impact of the drug resistance on the radioresistance in human pancreatic cancer cell lines. Methods Three drug resistant pancreatic caner cell sublines induced by fluorouracil (5-FU), adriamycin (ADM) and gemcitabine respectively, SW1990/FU, SW1990/ADM and SW1990/Gz, were tested for the cell cycle and radio-sensitivity with flow cytometry and clonogenic assay. Results Compared with SW1990, the cell cycle assay indicated higher G0/G1 period percentage in SW1990/FU and SW1990/Gz, but the G2/M period percentage decreased; SW1990/FU had the same while SW1990/Gz had lower S period percentage. SW1990/ADM almost had a similar cell cycle with SW1990. Clonogenic assay showed both SW1990/FU and SW1990/Gz had greater survival fraction (SF2) than SW1990, but SW1990/ADM had seemingly similar SF2 as SW1990. Conclusion Drug resistant pancreatic cancer cell lines have reduced G2/M period percentage and increased radioresistance.

Key concepts: Radioresistance, Clonogenic assay, Medicine, Cell cycle, Cancer research, Flow cytometry, Oncology, Internal medicine

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