The Study in Lasting Effectivity on Conditionally Replicating Adenovirus shRNA Mediating Survivin Gene Silencing in Colon Carcinoma Cell Line HT-29

Objective To investigate the lasting effect of conditionally replicating adenovirus shRNA on survivin gene silencing in colon carcinoma cell lineHT-29. Methods We transfected the colon cancer HT-29 cell with Ad-delE1b55KD-shRNA/Survivin-EGFP (control shRNA vector was the replication defective adenovirus called as Ad-shRNA/Survivin-EGFP). The expressions of EGFP, survivin mRNA and protein in HT-29 were detected at 1st day, 7th day, 14th day and 28th day after cell transfection. Results The expression of EGFP in HT-29 was high at 7th day after cell transfection but survivin mRNA and protein expressions inhibited in each experiment group, among which Ad-delE1b55KD-shRNA/Survivin-EGFP group showed the most high EGFP expression; at 14th day, the expression of EGFP went down but the survivin mRNA and protein expressions up-regulated obviously in replication defective adenovirus group and liposome vector group, however EGPF expression in HT-29 cell was still high and the survivin mRNA and protein expressions were still inhibited in Ad-delE1b55KD-shRNA/Survivin-EGFP group; at 28th day after cell HT-29 transfected, the expression of EGPF and the expression inhibition of survivin mRNA and protein disappeared in Ad-shRNA/Survivin-EGFP and liposome groups, but it was not in Ad-delE1b55KD-shRNA/Survivin-EGFP group(P0.05). Conclusion Conditionally replicating adenovirus shRNA can mediate the survivin gene silencing with long-lasting effects on colon carcinoma cell lines HT-29.