2001•Europe PMC (PubMed Central)Requires access
Fused expression of hantaan virus M and S partial gene segments in E. coli
Wen Luo, Zhikai Xu, Yan Yan, Wu Xing an, Fanglin Zhang, Yong Liu, Qian Zhao, Wentao Bai, Wang Hai-tao
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Abstract
The prokaryotic fusion expression vector pGEX-4T2-G1S0.7 was constructed through connection of G1 gene segment encoded by M gene with 0.7kb segment of 5^terminal in S gene region from hantann virus 76-118 strain and cloned into pGEX-4T2. The expression of the fusion protein was induced in E. coli XL1-Blue, and then the expression product was analyzed by ELISA and Western blot. It was proved that the prokaryotic fusion expression vector pGEX-4T2-G1S0.7 was constructed successfully by restriction enzyme analysis. After IPTG induction, ELISA results showed that the fusion protein could bind specifically to the hantaan virus nucleoprotein specific mAb and glycoprotein specific mAb, and Western blot result exhibited a novel protein band with Mr more than 1×10~(5) which was encoded by G1S0.7 and GST gene.