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Detection of monosomy 7 or 7q- in cases of myelodysplastic syndrome

WU Yafan

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Abstract

Objective To investigate the value of interphase fluorescence in situ hybridization(FISH) in the detection of monosomy 7 (-7)or deletion of long arm of chromosome 7(7q-) in the cases of myelodysplastic syndrome (MDS). Methods Forty six cases of MDS and 10 normal controls were studied simultaneously by conventional karyotype analysis and interphase FISH technique using SpectrumRed directly labeled DNA specific probe for 7q32. Two hundred interphase cells were analyzed for each case and the cells with one red hybridization spot7% were regarded as positive. Results Three cases displayed -7/7q- by conventional cytogenetics(CC) and were confirmed by interphase FISH. Six cases in 43 cases who did not show -7/7q- by CC displayed -7/7q- by interphase FISH. Conclusion Interphase FISH is very useful for the detection of -7 or 7q- in MDS and it is more sensitive than CC.

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What this paper is about

Objective To investigate the value of interphase fluorescence in situ hybridization(FISH) in the detection of monosomy 7 (-7)or deletion of long arm of chromosome 7(7q-) in the cases of myelodysplastic syndrome (MDS). Methods Forty six cases of MDS and 10 normal controls were studied simultaneously by conventional karyotype analysis and interphase FISH technique using SpectrumRed directly labeled DNA specific probe for 7q32. Two hundred interphase cells were analyzed for each case and the cells with one red hybridization spot7% were regarded as positive. Results Three cases displayed -7/7q- by conventional cytogenetics(CC) and were confirmed by interphase FISH. Six cases in 43 cases who did not show -7/7q- by CC displayed -7/7q- by interphase FISH. Conclusion Interphase FISH is very useful for the detection of -7 or 7q- in MDS and it is more sensitive than CC.

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Available abstract

Objective To investigate the value of interphase fluorescence in situ hybridization(FISH) in the detection of monosomy 7 (-7)or deletion of long arm of chromosome 7(7q-) in the cases of myelodysplastic syndrome (MDS). Methods Forty six cases of MDS and 10 normal controls were studied simultaneously by conventional karyotype analysis and interphase FISH technique using SpectrumRed directly labeled DNA specific probe for 7q32. Two hundred interphase cells were analyzed for each case and the cells with one red hybridization spot7% were regarded as positive. Results Three cases displayed -7/7q- by conventional cytogenetics(CC) and were confirmed by interphase FISH. Six cases in 43 cases who did not show -7/7q- by CC displayed -7/7q- by interphase FISH. Conclusion Interphase FISH is very useful for the detection of -7 or 7q- in MDS and it is more sensitive than CC.

Key concepts: Interphase, Fluorescence in situ hybridization, Monosomy, Chromosome 7 (human), Fish <Actinopterygii>, Karyotype, Myelodysplastic syndromes, Cytogenetics

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Detection of monosomy 7 or 7q- in cases of myelodysplastic syndrome — Research Paper | ScholarLens