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2020•Research SquareOpen access

Identification of Differential mRNA and lncRNA Expression in AcMNPV- Infected Sf9 Cells

Tiejun Zhao, Riqiang Deng, Mengqiu Chen, Xunzhang Wang

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Abstract

Abstract Autographa californica multiple Nucleopolyhedrovirus (AcMNPV) is the archetypal species of the alphabaculovirus. Sf9 are the ovarian cells of their host—Spodoptera frugiperda. In this study, a total of 3,463 pieces of time-series differentially expressed RNA are identified, including 1,200 mRNA and 2,263 lncRNA, with high-throughput sequencing technology using samples collected from Sf9 cells at different time points after AcMNPV infection. The result could provide a reference for the further study of the interaction and regulatory mechanism between the virus and the host.

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Abstract Autographa californica multiple Nucleopolyhedrovirus (AcMNPV) is the archetypal species of the alphabaculovirus. Sf9 are the ovarian cells of their host—Spodoptera frugiperda. In this study, a total of 3,463 pieces of time-series differentially expressed RNA are identified, including 1,200 mRNA and 2,263 lncRNA, with high-throughput sequencing technology using samples collected from Sf9 cells at different time points after AcMNPV infection. The result could provide a reference for the further study of the interaction and regulatory mechanism between the virus and the host.

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Available abstract

Abstract Autographa californica multiple Nucleopolyhedrovirus (AcMNPV) is the archetypal species of the alphabaculovirus. Sf9 are the ovarian cells of their host—Spodoptera frugiperda. In this study, a total of 3,463 pieces of time-series differentially expressed RNA are identified, including 1,200 mRNA and 2,263 lncRNA, with high-throughput sequencing technology using samples collected from Sf9 cells at different time points after AcMNPV infection. The result could provide a reference for the further study of the interaction and regulatory mechanism between the virus and the host.

Key concepts: Sf9, Autographa californica, Spodoptera, Biology, Messenger RNA, Virology, RNA, Host (biology)

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