Studies on microbial glycerol dehydrogenase. Part IV. Glycerol dehydrogenase and glycerol dissimilation in Cellulomonas sp. NT3060.
Hiroshi Nishise, Atsuko Ogawa, Yoshiki Tani, Hideaki Yamada
Abstract
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Hiroshi Nishise, Atsuko Ogawa, Yoshiki Tani, Hideaki Yamada
Abstract
Open-access reader
Glycerol kinase and glycerol 3-phosphate (G3P) dehydrogenase activities were induced when Cellulomonas sp.NT3060, a high level producer of glycerol dehydrogenase, was grown on either glycerol or G3P.Eleven glycerol-negative mutants were isolated from the wild type strain.These mutant strains were classified into three groups which are defective in glycerol kinase, G3P dehydrogenase and both the enzymes, respectively.All the mutant strains were unable to grow on glycerol, but still showed high glycerol dehydrogenase activity.Gluconate enhanced the formation
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Glycerol kinase and glycerol 3-phosphate (G3P) dehydrogenase activities were induced when Cellulomonas sp.NT3060, a high level producer of glycerol dehydrogenase, was grown on either glycerol or G3P.Eleven glycerol-negative mutants were isolated from the wild type strain.These mutant strains were classified into three groups which are defective in glycerol kinase, G3P dehydrogenase and both the enzymes, respectively.All the mutant strains were unable to grow on glycerol, but still showed high glycerol dehydrogenase activity.Gluconate enhanced the formation
Key concepts: Glycerol kinase, Glycerol, Dehydrogenase, Biochemistry, Chemistry, Branched-chain alpha-keto acid dehydrogenase complex, Mutant, Enzyme