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2005•Unpublished venueRequires access

Effects of berberine on adiponectin mRNA expression in 3T3-L1 adipocyte

Haiying Hu

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Abstract

Objective: To explore the effects of berberine and insulin on adiponectin mRNA expression in 3T3-L1 adipocyte. Method:The 3T3-L1 adipocyte was treated with berberine and insulin for 48 hours,the level of adiponectin mRNA in 3T3-L1 adipocyte expression was determined with semiquantity RT-PCR using β-actin as internal reference. Result: The level of adiponetin mRNA in 3T3-L1 adipocyte was increased after treated with berberine only(P0.05),the effect of berberine was inhibited by high concentration insulin(P0.05). Conclusion: in vitro,berberine increases the expression of adiponectin in 3T3-L1 adipocyte,insulin inhibits the effect of berberine.

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What this paper is about

Objective: To explore the effects of berberine and insulin on adiponectin mRNA expression in 3T3-L1 adipocyte. Method:The 3T3-L1 adipocyte was treated with berberine and insulin for 48 hours,the level of adiponectin mRNA in 3T3-L1 adipocyte expression was determined with semiquantity RT-PCR using β-actin as internal reference. Result: The level of adiponetin mRNA in 3T3-L1 adipocyte was increased after treated with berberine only(P0.05),the effect of berberine was inhibited by high concentration insulin(P0.05). Conclusion: in vitro,berberine increases the expression of adiponectin in 3T3-L1 adipocyte,insulin inhibits the effect of berberine.

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Available abstract

Objective: To explore the effects of berberine and insulin on adiponectin mRNA expression in 3T3-L1 adipocyte. Method:The 3T3-L1 adipocyte was treated with berberine and insulin for 48 hours,the level of adiponectin mRNA in 3T3-L1 adipocyte expression was determined with semiquantity RT-PCR using β-actin as internal reference. Result: The level of adiponetin mRNA in 3T3-L1 adipocyte was increased after treated with berberine only(P0.05),the effect of berberine was inhibited by high concentration insulin(P0.05). Conclusion: in vitro,berberine increases the expression of adiponectin in 3T3-L1 adipocyte,insulin inhibits the effect of berberine.

Key concepts: Berberine, Adiponectin, 3T3-L1, Adipocyte, Insulin, Internal medicine, Endocrinology, Chemistry

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Effects of berberine on adiponectin mRNA expression in 3T3-L1 adipocyte — Research Paper | ScholarLens