Total Phenolic Content and Antioxidant Activities of Blueberry Pomace Extracts

Total phenolics and antioxidant activities of blueberry pomace extracts were studied in the present study.Water,40% ethanol and cellulase-ultrasonic-assisted extraction were used to extract blueberry pomace in this study.Folin-Ciocalteu method was employed for total phenol contents determination.Moreover,the antioxidant activities of aqueous extracts,ethanol extracts and cellulase-ultrasonic-assisted extracts were determined by DPPH free radical scavenging assay and O—·2 free radical scavenging assay.Results showed that total phenolic content of cellulase-ultrasonic-assisted extracts(425.36±15.2 mg GAE·100 g-1 DW) was much higher than that of aqueous extracts(169.46±9.7 mg GAE·100 g-1 DW) and ethanol extracts(218.39±12.5 mg GAE·100 g-1 DW).The antioxidant activity of cellulase-ultrasonic-assisted extracts in the DPPH radical scavenging and superoxide radical scavenging assay were 2.67±0.13 g Vc·100 g-1 DW and 2.48±0.14 g Vc·100 g-1 DW,respectively.These values were much lower than those of the aqueous extracts and ethanol extracts.