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Structural conformation of prokaryotic plasmid expressing the gp41 of HIV-1 SF_2 strain

Lu Xiao

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Abstract

Objective To clone and express HIV gp41 in E.coli. Method The purified env gene derived from HIV SF 2 strain genome by PCR was recombined with pBV220 which served as high efficiency expression vector and expressed gp41 in E.coli HB101 strain. Results There was a dye band equal to 44 000 displayed by SDS PAGE.It was confirmed that the protein of 44 000 can specially react with HIV 1 positive serum by using Western blot. Conclusion The recombinant protein can be regarded as HIV gp41.

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What this paper is about

Objective To clone and express HIV gp41 in E.coli. Method The purified env gene derived from HIV SF 2 strain genome by PCR was recombined with pBV220 which served as high efficiency expression vector and expressed gp41 in E.coli HB101 strain. Results There was a dye band equal to 44 000 displayed by SDS PAGE.It was confirmed that the protein of 44 000 can specially react with HIV 1 positive serum by using Western blot. Conclusion The recombinant protein can be regarded as HIV gp41.

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Available abstract

Objective To clone and express HIV gp41 in E.coli. Method The purified env gene derived from HIV SF 2 strain genome by PCR was recombined with pBV220 which served as high efficiency expression vector and expressed gp41 in E.coli HB101 strain. Results There was a dye band equal to 44 000 displayed by SDS PAGE.It was confirmed that the protein of 44 000 can specially react with HIV 1 positive serum by using Western blot. Conclusion The recombinant protein can be regarded as HIV gp41.

Key concepts: Gp41, Strain (injury), Recombinant DNA, Human immunodeficiency virus (HIV), clone (Java method), Western blot, Plasmid, Molecular biology

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