Effects of Proteasome Inhibitor MG132 on Cell Proliferation,Apoptosis,and Cell Cycle in HEC-1B and Ishikawa Cells

Objective To study the anti-cancer activities of Z-Leu-Leu-Leu-cho(MG132) against human endometrial carcinoma HEC-1B and Ishikawa cells and the potential of MG132 in human endometrial cancer therapy.Methods HEC-1B and Ishikawa cells were treated with MG132.Cell proliferation was assessed by MTT while cell apoptosis rate and cell-cycle distribution were assessed by flow cytometry.Results The proliferation of HEC-1B and Ishikawa cells was inhibited by MG132 and cell proliferation was significantly inhibited with the raised concentration of MG132(P0.01),Ishikawa cells were more sensitive than HEC-1B cells to MG132.MG132 could induce the apoptosis of HEC-1B and Ishikawa cells(P=0.000).Cell cycle analysis indicated that the percentage of HEC-1B cells was increased in G2 phase(P0.05) while Ishikawa cells' was increased in G1 and G2 phase(P0.05).Conclusion Proteasome inhibitor MGl32 could inhibit the proliferation,promote cell apoptosis,and block the cell cycle of HEC-1B and Ishikawa cells.MG132 may be a potential treatment for endometrial cancer.