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2010•Unpublished venueRequires access

Isolation and characterization of 20 polymorphic microsatellite markers in Macrobrachium nipponense

Yan ZhaoHui, WangXiangshan Ji, Yongqing ZengPingping, YangLei Ding

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Abstract

We developed 20 polymorphic microsatellite loci by 5 0 -anchored PCR and constructing a microsatellite- enriched genomic library. The number of alleles per locus ranged from two to twelve, and observed and expected heterozygosities from 0.2357 to 1.0000 and from 0.4156 to 0.9169, respectively. Seven loci deviated from the HWE in the sampled population, null alleles were found in four loci, and gamete linkage disequilibrium between two loci was significant. These microsatellite markers will be useful for the study of population structure and genetic diversity of M. nipponense.

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What this paper is about

We developed 20 polymorphic microsatellite loci by 5 0 -anchored PCR and constructing a microsatellite- enriched genomic library. The number of alleles per locus ranged from two to twelve, and observed and expected heterozygosities from 0.2357 to 1.0000 and from 0.4156 to 0.9169, respectively. Seven loci deviated from the HWE in the sampled population, null alleles were found in four loci, and gamete linkage disequilibrium between two loci was significant. These microsatellite markers will be useful for the study of population structure and genetic diversity of M. nipponense.

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Available abstract

We developed 20 polymorphic microsatellite loci by 5 0 -anchored PCR and constructing a microsatellite- enriched genomic library. The number of alleles per locus ranged from two to twelve, and observed and expected heterozygosities from 0.2357 to 1.0000 and from 0.4156 to 0.9169, respectively. Seven loci deviated from the HWE in the sampled population, null alleles were found in four loci, and gamete linkage disequilibrium between two loci was significant. These microsatellite markers will be useful for the study of population structure and genetic diversity of M. nipponense.

Key concepts: Microsatellite, Linkage disequilibrium, Biology, Genetics, Locus (genetics), Allele, Null allele, Population

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Isolation and characterization of 20 polymorphic microsatellite markers in Macrobrachium nipponense — Research Paper | ScholarLens