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2013•Shiyong kouqiang yixue zazhiRequires access

A preliminary study on the expression and mechanism of Toll-like receptor 4 in RAW264.7 cells

Lu Qu

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Abstract

Objective: To study the expression of Toll like receptor 4(TLR4) in RAW 264.7 cells and its biological effects on the cells.Methods: RAW264.7 cells were stimulated with different concentrations of LPS,cell migration assay,RT-PCR and Western blot were used to study the migration of the cells,the mRNA and protein expression of TLR4 and mRNA expression of MMP9,TRAP and cathinK in RAW264.7 cells respectively.Results: TLR4 was expressed in RAW264.7 cells,the mRNA and protein expression levels were increased by LPS in a dose-dependant manner.The cell migration rate was increased and the mRNA expression levels of MMP9,TRAP and cathinK were increased by LPS treatment.Conclusion: LPS may activate TLR4 in RAW264.7 cells.The activation of TLR4 can increase the migration rate and the related enzyme mRNA level in RAW264.7 cells.

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What this paper is about

Objective: To study the expression of Toll like receptor 4(TLR4) in RAW 264.7 cells and its biological effects on the cells.Methods: RAW264.7 cells were stimulated with different concentrations of LPS,cell migration assay,RT-PCR and Western blot were used to study the migration of the cells,the mRNA and protein expression of TLR4 and mRNA expression of MMP9,TRAP and cathinK in RAW264.7 cells respectively.Results: TLR4 was expressed in RAW264.7 cells,the mRNA and protein expression levels were increased by LPS in a dose-dependant manner.The cell migration rate was increased and the mRNA expression levels of MMP9,TRAP and cathinK were increased by LPS treatment.Conclusion: LPS may activate TLR4 in RAW264.7 cells.The activation of TLR4 can increase the migration rate and the related enzyme mRNA level in RAW264.7 cells.

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Available abstract

Objective: To study the expression of Toll like receptor 4(TLR4) in RAW 264.7 cells and its biological effects on the cells.Methods: RAW264.7 cells were stimulated with different concentrations of LPS,cell migration assay,RT-PCR and Western blot were used to study the migration of the cells,the mRNA and protein expression of TLR4 and mRNA expression of MMP9,TRAP and cathinK in RAW264.7 cells respectively.Results: TLR4 was expressed in RAW264.7 cells,the mRNA and protein expression levels were increased by LPS in a dose-dependant manner.The cell migration rate was increased and the mRNA expression levels of MMP9,TRAP and cathinK were increased by LPS treatment.Conclusion: LPS may activate TLR4 in RAW264.7 cells.The activation of TLR4 can increase the migration rate and the related enzyme mRNA level in RAW264.7 cells.

Key concepts: TLR4, Messenger RNA, Western blot, Toll-like receptor, Receptor, MMP9, Chemistry, Molecular biology

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