Prokaryotic Expression of Xinjiang Strain HPV16 E6 Gene

A 450bp HPV16 E6 DNA fragment was amplified by using primers designed from the coding sequence of HPV16 E6 gene by PCR. The fragment containing the coding sequence of HPV16 E6 protein was cloned into vector pMD 18T ( pMD T HPV16E6) and transformed into E.coli JM109. Positive clone was identified successfully by enzyme digestion. The 450bp HPV16E6 DNA fragment was digested with BamH I and Sal I and subcloned into vector pET28a(+), forming the recombinant plasmid of pET28 HPV16E6.After induction with IPTG, the pET28HPV16E6 expressed a 18kD E6 protein in E.coli DL21(DE3).The result showed that HPV16 E6 gene can be expressed in E.coli in the form of inclusion body at a high yield.