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Comparative study on ecotype differentiation of Leymus chinensis in different geographic populations at moleculat level

Ma Yu

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Abstract

The Leymus chinensis from different geographic populations were cultured in the campus and were used as materials in this research.Random amplified polymorphic DNA(RAPD) analysis was applied to these populations.Twenty random 10 mer primers yielded one hundred RAPD polymophic bands.Bands were scored (+/-) and un weighed pair group method with arithmetic mean(UPGMA) was used to generate dendrograms showing genetic relationships among the evaluated L.chinensis populations.Cluster analysis showed that Xiwuqi and Dingweizhan's populations have the smallest genetic distance and belong to the same ecotype;Xeltala and Jilin's may belong to the different ecotypes;Aqi's may be thought as other ecotype,because it has special characteristics.The result also made us sure that water was the most important factor among all that influence the ecotype differentiation among different geographic populations of Leymus chinensis .

About this research paper

What this paper is about

The Leymus chinensis from different geographic populations were cultured in the campus and were used as materials in this research.Random amplified polymorphic DNA(RAPD) analysis was applied to these populations.Twenty random 10 mer primers yielded one hundred RAPD polymophic bands.Bands were scored (+/-) and un weighed pair group method with arithmetic mean(UPGMA) was used to generate dendrograms showing genetic relationships among the evaluated L.chinensis populations.Cluster analysis showed that Xiwuqi and Dingweizhan's populations have the smallest genetic distance and belong to the same ecotype;Xeltala and Jilin's may belong to the different ecotypes;Aqi's may be thought as other ecotype,because it has special characteristics.The result also made us sure that water was the most important factor among all that influence the ecotype differentiation among different geographic populations of Leymus chinensis .

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Available abstract

The Leymus chinensis from different geographic populations were cultured in the campus and were used as materials in this research.Random amplified polymorphic DNA(RAPD) analysis was applied to these populations.Twenty random 10 mer primers yielded one hundred RAPD polymophic bands.Bands were scored (+/-) and un weighed pair group method with arithmetic mean(UPGMA) was used to generate dendrograms showing genetic relationships among the evaluated L.chinensis populations.Cluster analysis showed that Xiwuqi and Dingweizhan's populations have the smallest genetic distance and belong to the same ecotype;Xeltala and Jilin's may belong to the different ecotypes;Aqi's may be thought as other ecotype,because it has special characteristics.The result also made us sure that water was the most important factor among all that influence the ecotype differentiation among different geographic populations of Leymus chinensis .

Key concepts: Ecotype, UPGMA, RAPD, Leymus, Biology, Dendrogram, Botany, Edaphic

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