2000•中国科学通报：英文版Requires access
Cloning and expression of a novel human HCUTA cDNA
Wen-Qin, Luo, Jian-he, Chen, Haijun, Zhou, Xiaowei Xiaowei, Huang, Yan, Jiangang, Yuan, Boqin, Qiang Qiang
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Abstract
Copper is one of the most important trace elements to life. Human HCUTA is a novel cDNA encoding a 156aa protein, which may participate in human copper tolerance system. The HCUTA protein is highly similar to protein CUT A1 of E. coli. The whole opening reading frame of HCUTA cDNA was amplified by PCR and cloned into pET28a + express vector, and the HCUTA protein was effectively expressed in E. co/i BL21 (DE3).