2002•Nanjing Linye Daxue xuebaoRequires access
Purification and Characterization of Xylanases from Trichoderma reesei
Mao Lian-shan, Xiangyang Song, Qiang Yong, Yang Fu-guo, Chuncai Yao, Shiyuan Yu
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Abstract
Two parts of xylanases (Part A and Part B) were separated and purified from a culture filtrate of Trichoderma reesei Rut C-30 by ammonium sulfate precipitation, followed by DEAE-sephadex A-5- and SP-Sephadex C-50 column chromatography. Part A and Part B were further purified to homogeneity by Sephadex G-100 column chromatography. The molecular weights of Part A and Part B were estimated to be 20300 and 13500 by SDS-PAGE chromatography. The optimal reaction conditions for Part A and Part B were at 45℃, pH4.5, and at 55℃, pH5.5, respectively. Part A was stable in a pH range from 3.0 to 5.5, while Part B was stable in pH range from 3.5 to 7.5. The major products of enzymatic hydrolysis with Part A were xylo-oligosacchrides, with a small amount of xylose, while that with Part B were merely xylo-oligosacchrides.