Interaction of vascular endothelial growth factor-C over-expression tongue squamous cell carcinoma cell line Tca8113 with peri carcinoma lymphatics

Objective To detect the role of Vascular endothelial growth factor C(VEGF C) in the interaction of tongue squamous cell carcinoma (TSCC) with the peri carcinoma lymphatics.Methods TSCC cells were implanted on the chorioallantoic membrane (CAM), in which, group A was transfected by plasmid pREP7 including VEGF C, group B was transfected by plasmid pREP7, and group C was Tca8113. Lymphatic vessels were stained by 5′ Nase.Morphologic analysis was used to evaluate the alteration of lymphatic vessels density (LVD), area and perimeter.Results The transplanted tumor well on CAM. The expression of VEGF C in group A was higher than that in control group B and C. The LVD, perimeter and area in group A were (5 3±0 41)/high power field, (148±21) μm and (76 8±13 5)μm 2 respectively ingroup A, which were significantly higher than that of group B and C ( P 0 01), while there were no significant difference between group B and C.Conclusions CAM is ideal model for the research of lymphatic vessels; Over expression of VEGF C could induce the dilatation and LVD increase of peri tumor lymphatic vessels, which maybe one of the mechanisms that VEGF C metastasis of TSCC through lymphatic vessels.