2015•生物医学与环境科学：英文版Requires access

Visual Detection of Murray Valley Encephalitis Virus by Reverse Transcription Loop-Mediated Isothermal Amplification

Gong, Rui Rui, WANG Wang, Han, Hua, Liang Qin, Hồng, Guo, Xiao Xiao, Ping Ping, M. A, Xue, Jun Jun

Abstract

A sensitive reverse transcription loop-mediated isothermal amplification(RT-LAMP)assay was developed for rapid visual detection of Murray valley encephalitis virus(MVEV)infection.The reaction was performed in one step in a single tube at 63°C for 60 min with the addition of the hydroxynaphthol blue(HNB)dye prior to amplification.The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold

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Key concepts: Reverse Transcription Loop-mediated Isothermal Amplification, Loop-mediated isothermal amplification, Reverse transcriptase, Detection limit, Virology, Molecular biology, Virus, Reverse transcription polymerase chain reaction