Genetic structure of different natural Osmanthus fragrans populations based on AFLP method

Osmanthus fragrans is being concerned in recent years due to its high economic value and ornamental value. It can provide significant evidences for breeding of new cultivars and protecting of natural populations with studying on the genetic diversity of natural populations of O. fragrans. The genetic diversity of 96 O. fragrans individuals of two natural populations from Quannan County of Jiangxi Province and Changting County of Fujian Province was estimated using amplified fragment length polymorphism(AFLP) method. A total number of 330 reproducible bands were amplified using seven AFLP primer combinations, and 276 bands were polymorphic with a proportion of 83.64%. At the species level, Shannon's information index(I) was 0.428 3, the Nei's genetic diversity(He) was 0.285 6, which indicated the genetic diversity of O. fragrans was rich; at the population level, Shannon's information index and Nei's genetic diversity of Fujian population was higher 1.5 than Jiangxi population, that showed Fujian population owned much rich genetic resource. Moreover, AMOVA indicated the most total genetic variation was within populations with a proportion of 71% and the less was among the populations with a proportion of 29%. Meanwhile, a certain extent level of genetic differentiation(Gst =0.161 6) was detected among the populations and genetic flow was 2.594 9.