The variance analysis of immune responses between different HBV antigens and different types of T cells in HBV-infected people

To explore the characteristics and differences of different types of hepatitis B virus(HBV) infection by observing immune reponse between T cell and HBV antigen protein, 107 cases of HBV infection patients were divided into HBV carrier group A(n=32), acute hepatitis group B(n=38), chronic hepatitis group C(n =37), with previous HBV patients(n=41) as controls. T cells and the immune response of HBeAg, HBcAg, HBsAg of 4 groups were analyzed by immune fluorescence analyzer. General situation analysis showed that ALT, AST, and total bilirubin levels in patients with acute hepatitis B and HBV DNA content in patients with chronic hepatitis B were significantly higher than those in other groups(P 0.05). While response frequency analysis illustrated that positive reaction frequency of three common HBV antigens has no statistical difference in patients with acute hepatitis B and chronic hepatitis B; HBV carrier group had a significantly higher response frequency of HBeAg than HBcAg and HBsAg(P 0.05); previous HBV group had a significantly lower response frequency of HBsAg than HBcAg and HBeAg(P 0.05). Reaction intensity analysis showed that the reaction intensity of HBeAg in acute hepatitis group and HBV carrier group was the highest, the reaction intensity of HBcAg in chronic hepatitis group and control group was the highest, while the reaction intensity of HBsAg in acute hepatitis group was the highest than the rest of the group(P 0.05). We conclude that the detection of T cell responses to hepatitis B virus antigen protein may predict the development of the patient's condition, and thus be used as guidance for HBV diagnosis.