Comparative Study on Resistant Starch Structure of Resistant Starch Enriched Rice Mutants
Jiao Guiai, Shaoqing Tang, Luo Ju, Peisong Hu, Dapo Box
Abstract
Jiao Guiai, Shaoqing Tang, Luo Ju, Peisong Hu, Dapo Box
Abstract
Two resistant starch enriched rice mutants RS111,AE and the wild type rice R7954 and IR36 were compared in the resistant starch structure by using gel permeation chromatography and capillary electrophoresis.The main component of the resistant starch in RS111 was amylose,the ratio of amylose to amylopectin was much higher compared to the wild type R7954,and the resistant starch amylopectin chain length distribution in RS111 was similar to the wild type,only the proportion of short chains of DP(degree of polymerization)=10 was decreased;Howerver,for the resistant starch in AE,the proportion of amylose and amylopectin were almost equal,amylose and amylopectin were both increased,and amylose to amylopectin ratio was not distinctively different from the wild type IR36,but the amylopectin chain length distribution was much different from the wild type,the proportion of short chains of DP=10 was much higher,but the proportion of chains of DP= 16 and 21 was much lower compared with the wild type IR36.
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Two resistant starch enriched rice mutants RS111,AE and the wild type rice R7954 and IR36 were compared in the resistant starch structure by using gel permeation chromatography and capillary electrophoresis.The main component of the resistant starch in RS111 was amylose,the ratio of amylose to amylopectin was much higher compared to the wild type R7954,and the resistant starch amylopectin chain length distribution in RS111 was similar to the wild type,only the proportion of short chains of DP(degree of polymerization)=10 was decreased;Howerver,for the resistant starch in AE,the proportion of amylose and amylopectin were almost equal,amylose and amylopectin were both increased,and amylose to amylopectin ratio was not distinctively different from the wild type IR36,but the amylopectin chain length distribution was much different from the wild type,the proportion of short chains of DP=10 was much higher,but the proportion of chains of DP= 16 and 21 was much lower compared with the wild type IR36.
Key concepts: Amylopectin, Amylose, Starch, Resistant starch, Degree of polymerization, Chemistry, Gel permeation chromatography, Polysaccharide