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PURIFICATION AND PROPERTIES OF β-GLUCANASE PRODUCED FROM STRAIN GXC OF TRICHODERMA REESEI

Sun Jiang

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Abstract

The β-glucanase from strain GXC of Trichoderma reesei was purified in three steps which comprised ammonium sulfate precipitation, Sephadex G-100 chromatography, and DEAE-Sephadex A-50 chromatography. The purified enzyme showed an activity increase of 14.60 fold, and an activity recovery of 6.62%. The optimal temperature and pH of the enzyme were 60℃ and 5.0，respectively . The β-glucanase was more stable at low pH than at high pH, and was relatively stable below 60℃ . Cu～2+, Mn～2+, Mg～2+, Fe～3+ and K+ had inhibitory effect on the enzyme activity; Zn～2+, Ca～2+, Co～2+ and Fe～2+ could stimulate the activity.

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What this paper is about

The β-glucanase from strain GXC of Trichoderma reesei was purified in three steps which comprised ammonium sulfate precipitation, Sephadex G-100 chromatography, and DEAE-Sephadex A-50 chromatography. The purified enzyme showed an activity increase of 14.60 fold, and an activity recovery of 6.62%. The optimal temperature and pH of the enzyme were 60℃ and 5.0，respectively . The β-glucanase was more stable at low pH than at high pH, and was relatively stable below 60℃ . Cu～2+, Mn～2+, Mg～2+, Fe～3+ and K+ had inhibitory effect on the enzyme activity; Zn～2+, Ca～2+, Co～2+ and Fe～2+ could stimulate the activity.

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Available abstract

The β-glucanase from strain GXC of Trichoderma reesei was purified in three steps which comprised ammonium sulfate precipitation, Sephadex G-100 chromatography, and DEAE-Sephadex A-50 chromatography. The purified enzyme showed an activity increase of 14.60 fold, and an activity recovery of 6.62%. The optimal temperature and pH of the enzyme were 60℃ and 5.0，respectively . The β-glucanase was more stable at low pH than at high pH, and was relatively stable below 60℃ . Cu～2+, Mn～2+, Mg～2+, Fe～3+ and K+ had inhibitory effect on the enzyme activity; Zn～2+, Ca～2+, Co～2+ and Fe～2+ could stimulate the activity.

Key concepts: Trichoderma reesei, Ammonium sulfate precipitation, Sephadex, Glucanase, Chemistry, Ammonium sulfate, Enzyme, Enzyme assay

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PURIFICATION AND PROPERTIES OF β-GLUCANASE PRODUCED FROM STRAIN GXC OF TRICHODERMA REESEI — Research Paper | ScholarLens