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2002•Journal of LeukemiaRequires access

PRELIMINARY STUDIES ON THE EFFECT OF ARSENIC TRIOXIDE ON PROLIFERATION AND APOPTOSIS OF LEUKEMIC CELLS AND IT′S MECHANISM

Yun Wang

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Abstract

Objective:To investigate the effect of arsenic trioxide (As 2O 3) on proliferation,cell cycle regulation and apoptosis in leukemic cell lines and it's mechanism.Methods:MTT assay,electrophoresis of genomic DNA,as well as protein/DNA multiparameter flow cytometry were used.Results:Cell proliferation were significantly inhibited in a dose and time dependent manner.Apoptosis were induced in these cells.Furthermore,As 2O 3 could change the content of some cell cycle and apoptosis related proteins in NB 4/Jurkat cells in which both G 1 and G 2/M phases were arrested.Conclusion:The change of all cycle regulatory protein may play an improtant role in proliferation inhibition and apoptosis of leukemic cells regulatery from As 2O 3.

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Objective:To investigate the effect of arsenic trioxide (As 2O 3) on proliferation,cell cycle regulation and apoptosis in leukemic cell lines and it's mechanism.Methods:MTT assay,electrophoresis of genomic DNA,as well as protein/DNA multiparameter flow cytometry were used.Results:Cell proliferation were significantly inhibited in a dose and time dependent manner.Apoptosis were induced in these cells.Furthermore,As 2O 3 could change the content of some cell cycle and apoptosis related proteins in NB 4/Jurkat cells in which both G 1 and G 2/M phases were arrested.Conclusion:The change of all cycle regulatory protein may play an improtant role in proliferation inhibition and apoptosis of leukemic cells regulatery from As 2O 3.

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Available abstract

Objective:To investigate the effect of arsenic trioxide (As 2O 3) on proliferation,cell cycle regulation and apoptosis in leukemic cell lines and it's mechanism.Methods:MTT assay,electrophoresis of genomic DNA,as well as protein/DNA multiparameter flow cytometry were used.Results:Cell proliferation were significantly inhibited in a dose and time dependent manner.Apoptosis were induced in these cells.Furthermore,As 2O 3 could change the content of some cell cycle and apoptosis related proteins in NB 4/Jurkat cells in which both G 1 and G 2/M phases were arrested.Conclusion:The change of all cycle regulatory protein may play an improtant role in proliferation inhibition and apoptosis of leukemic cells regulatery from As 2O 3.

Key concepts: Arsenic trioxide, Apoptosis, Cell cycle, Jurkat cells, Flow cytometry, Cell growth, Chemistry, Cell biology

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PRELIMINARY STUDIES ON THE EFFECT OF ARSENIC TRIOXIDE ON PROLIFERATION AND APOPTOSIS OF LEUKEMIC CELLS AND IT′S MECHANISM — Research Paper | ScholarLens