Preliminary evidence that a hepatitis E virus(HEV)ORF_2 recombinant protein protects cynomolgus macaques against challenge with wild-type HEV

Objective To observe the protective effict of hepatitis E virus(HEV)ORF 2 recombinant protein expressed in prokaryote cell cynomolgus macaques(cynos)against challenging with wild-type HEV.Methods Cynos were immunized with HEV ORF 2 recombinant protein and then challenged with wild-type HEV,the unimmunized cynos were used as control.Blood samples were collected and tested to see if there were dynamic changes of ALT and antibody to HEV before and after challenge with wild-type HEV.Results All the five unimmunized cynos re-presented hepatitis 3 weeks after challenging with wild-type HEV.However,all the five immunized cynos showed no hepatitis and pathological changes.Conclusion Cynos can be efficiently protected by immunization with HEV ORF 2 recombinant protein against wild-type HEV.This protein can be a promising candidate for HEV vaccine.