Sirolimus inhibits the differentiation,proliferation and migration of endothelial progenitor cells in vitro

Objective To investigate the effect of sirolimus on differentiation,proliferation,adhesion and migration of endothelial progenitor cells(EPC)in vitro.Methods(1)Mononuclear cells(MNC)were isolated from rat bone marrow by Ficoll density gradient centrifugation and cultured on fibronectin-coated culture dishes with or without sirolimus(0.01-100 ng/ml)for 12 days.(2)After 8 days cultured,attached cells were treated with sirolimus(0.1-200 ng/ml)or vehicle for various time points(12 h,24 h,48 h and 96 h).EPC were identified as adherent cells double positive stained for FITC-UEA-Ⅰand DiI-acLDL under laser confocal immunofluence microscope.EPC proliferation,migration were assayed with MTT assay and modified Boyden chamber assay respectively.Results EPC number differentiated from MNC at 12 days was significantly lower in sirolimus treated cells in a dose-dependent manner than that of vehicle-treated cells.Sirolimus also significantly inhibited the proliferative,migratory and adhesive capacity of EPC in a time and dose dependent manner.Conclusion Present results suggested that sirolimus could inhibit EPC differentiation from MNC and reduce the proliferation,migration and adhesion capacities of EPC.