2002•Zhongguo nongye KexueRequires access
Studies of the Factors Affecting on Somatic Embryogenesis in Soybean
Ping Wang
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Abstract
The effects of the concentration of MS micro salts, 2,4 dichlorophenoxyacetic acid(2,4 D), naphthalene acetic acid (NAA), proline and adenine on callus formation and embryogenesis were investigated using orthogonal design with immature cotyledon of soybean. The results showed that the role of concentration of micro salts on frequency of callus formation and embryogenesis were significant. MS medium plused 2,4 D, NAA, proline or adenine can stimulate callus formation. The concentration of MS micro salts possessed obvious effects on somatic embryogenesis of soybeans. The category and concentration of the hormone needed were different among genotypes when embryogenesis are induced.