Effect of fluoride on the expression of Core-binding facorα1 in fibroblast and osteoblast

Objective To study the effects of fluoride on the expression of Core-binding facorα1 (cbfa1) in fibroblast and osteoblast. Methods Osteoblast and fbroblasts were exposed to different concentrations of fluoride (F-0,0.1,1.0,100.0,1 000.0,10 000.0,20 000.0μg/L). The levels of cbfa1 mRNA and protein at certain time were measured by using RT-PCR,ELISA and immunohistochemistry(IHC) methods. Results (1)In fibroblast, the content of cbfa1 protein increased obviously in groups of 0.1,1.0,100.0,10 000.0 and 20 000.0μg/L at 48 h[(2.35±0.08), (2.28±0.09), (2.32±0.09), (2.25±0.08), (2.28±0.09) ] and group of 10 000.0μg/L at 72 h (2.48±0.22). The level of cbfa1 mRNA was much higher in the group of 10 000.0μg/L at 48 h(1.29±0.30) than it was in the control (1.02±0.12). The stronger expression of cbfa1 was observed by IHC in fibroblast treated with 0.1μg/L for 48 h. (2) Comparing to the control, the content of cbfa1 protein increased in most of groups of fluoride-treated osteoblast. The expression of cbfa1 mRNA were higher in the groups of 0.1,1.0,100.0,1 000.0μg/L and much higher in group of 0.1μg/L (1.34±0.19) than it was in the control (1.27±0.20). Conclusions The expression of both mRNA and protein of cbfa1 were increased significantly in fibroblast and osteoblast treated with fluoride. This study suggested that fibroblast possibly play important role in the developing of extraperiosteal ossification of skeletal fluorosis.