Rapid detection method of three types of bacterial pathogens in aquatic products established by multiplex PCR

Objective To develop a rapid multiplex PCR(m-PCR) assay for simultaneously detection of three foodborne pathogens in aquatic products.Methods The invasion protein gene(invA) of Salmonella spp.,toxR gene(toxR) of Vibrio parahaemolyticus and invasion-associated protein p60 gene(iap) of Listeria monocytogenes were used as the gene targets.Results The multiplex PCR assay could be specific and rapid,and the detection limits were 10 cfu/ml when the artificially contaminated aquatic products were incubated at 37℃ for 10h.Conclusion The multiplex PCR assay developed in this study could provide a cost-effective supplement of conventional microbiological methods for routine monitoring of food.