2020Pakistan BioMedical JournalOpen access

Isolation of Plant Growth Promoting Bacteria from the Rhizosphere of Different Plants and Assessment of Their Plant Growth Promotion Potential

Anam Yousaf, Hassan Ahmed Khan, Tayyaba Younas

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Abstract

Plant are benefitted in different aspects by symbiotic bacteria. Environmental conditions, Plantconditions and type of pathogens determine these important services for plants Objective: Theresearch was conducted to assess the plant growth enhancing effects of wheat and cabbagerhizobacteria on the growth of wheat plant Methods: For this purpose, total 49 bacteria were isolatedand characterized from the rhizosphere of wheat and cabbage plants. The isolates were assessed forplant growth promoting properties such as: indole acetic acid production, phosphate solubilization,antibacterial activity and heavy metal resistance. Indole acetic acid was found to be produced by 7isolates and phosphate solubilization was shown by 20 isolates. Antibacterial activity was determinedagainst four clinical isolates like Staphylococcus aureus, Klebsiella sp., Escherichia coli and Pseudomonasaeruginosa Results: Antibacterial activity against Staphylococcus aureus was shown by 38 isolates, 12isolates showed antibacterial activity against Escherichia coli and Klebsiella sp., whereas no isolate wasfound to be positive against Pseudomonas aeruginosa. Another plant growth enhancing trait (heavymetal resistance) was shown by 28 rhizobacteria. In order to evaluate the capability of isolates toenhance the plant growth, bio-inoculation assay was performed using wheat seeds Conclusions:Rhizobacterial inoculation increased the number of roots, shoots, leaves and roots and shoot length ofwheat plantlets as compared to un-inoculated control.

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Plant are benefitted in different aspects by symbiotic bacteria. Environmental conditions, Plantconditions and type of pathogens determine these important services for plants Objective: Theresearch was conducted to assess the plant growth enhancing effects of wheat and cabbagerhizobacteria on the growth of wheat plant Methods: For this purpose, total 49 bacteria were isolatedand characterized from the rhizosphere of wheat and cabbage plants. The isolates were assessed forplant growth promoting properties such as: indole acetic acid production, phosphate solubilization,antibacterial activity and heavy metal resistance. Indole acetic acid was found to be produced by 7isolates and phosphate solubilization was shown by 20 isolates. Antibacterial activity was determinedagainst four clinical isolates like Staphylococcus aureus, Klebsiella sp., Escherichia coli and Pseudomonasaeruginosa Results: Antibacterial activity against Staphylococcus aureus was shown by 38 isolates, 12isolates showed antibacterial activity against Escherichia coli and Klebsiella sp., whereas no isolate wasfound to be positive against Pseudomonas aeruginosa. Another plant growth enhancing trait (heavymetal resistance) was shown by 28 rhizobacteria. In order to evaluate the capability of isolates toenhance the plant growth, bio-inoculation assay was performed using wheat seeds Conclusions:Rhizobacterial inoculation increased the number of roots, shoots, leaves and roots and shoot length ofwheat plantlets as compared to un-inoculated control.

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Available abstract

Plant are benefitted in different aspects by symbiotic bacteria. Environmental conditions, Plantconditions and type of pathogens determine these important services for plants Objective: Theresearch was conducted to assess the plant growth enhancing effects of wheat and cabbagerhizobacteria on the growth of wheat plant Methods: For this purpose, total 49 bacteria were isolatedand characterized from the rhizosphere of wheat and cabbage plants. The isolates were assessed forplant growth promoting properties such as: indole acetic acid production, phosphate solubilization,antibacterial activity and heavy metal resistance. Indole acetic acid was found to be produced by 7isolates and phosphate solubilization was shown by 20 isolates. Antibacterial activity was determinedagainst four clinical isolates like Staphylococcus aureus, Klebsiella sp., Escherichia coli and Pseudomonasaeruginosa Results: Antibacterial activity against Staphylococcus aureus was shown by 38 isolates, 12isolates showed antibacterial activity against Escherichia coli and Klebsiella sp., whereas no isolate wasfound to be positive against Pseudomonas aeruginosa. Another plant growth enhancing trait (heavymetal resistance) was shown by 28 rhizobacteria. In order to evaluate the capability of isolates toenhance the plant growth, bio-inoculation assay was performed using wheat seeds Conclusions:Rhizobacterial inoculation increased the number of roots, shoots, leaves and roots and shoot length ofwheat plantlets as compared to un-inoculated control.

Key concepts: Rhizobacteria, Rhizosphere, Shoot, Biology, Bacteria, Escherichia coli, Antibacterial activity, Inoculation

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