Evaluation of Microvessel Density in Odontogenic Keratocyst, Dentigerous Cyst and Radicular Cyst

Introduction: Odontogenic cysts are encountered relatively common in dental practice.Based on the new WHO classification, odontogenic cysts are categorized into 2 main groups that imitate their pathogenesis.Angiogenesis is the physiological process involving the growth of new blood vessels from pre-existing vessels and is a complex multi-stage process including degradation of the extracellular matrix, proliferation and migration of endothelial cells, capillary differentiation and anastomosis CD34 molecule is a cluster differentiation molecule present on certain cells within the human body.Objectives: Hence; the present study was conducted for analyzing the immunohistochemical expression of CD34 in odontogenic keratocyst, Radicular cyst and Dentigerous cyst.Methods: A total of 30 cases were included and were divided as follows: 10 cases of Periapical cyst, 10 cases of dentigerous cyst and 10 cases of Odontogenic cyst.The biopsy was obtained with the patient's consent under local anaesthesia.The specimens were immediately fixed in 10% neutral buffered formalin, processed and embedded in paraffin wax.Serial sections of4 µm were obtained from both the archival material and also from the cases.One group of sections was stained for routine haematoxylin and eosin examination to confirm the clinical diagnosis and also for comparison.The other group of sections was used for immunohistochemical study using mouse monoclonal Anti-vimentin antibody.The histomorphometric analysis of blood vessels was carried out by Moticplus software was attached with an Olympus BX41 microscope.The histomorphometric analysis of blood vessels was carried out by Image J software.Results: In the periapical cyst mean MVD was found to be 43.40 ± 6.851.Among the dentigerous cyst, mean MVD was 38.40 ± 17.141.P-value was found to be 0.30 found to be significant.In the odontogenickerato cyst mean MVD was found to be 57.20±27.59.Among the dentigerous cyst mean MVD was 38.40 ± 17.141.The mean difference in MVD between periapical cyst and odontogenickerato cyst was 18.800.Conclusion: CD34 expression act as an important role in determining the microvessel density of odontogenic cysts and it might relate to the invasive growth of the odonto genickeratocysts for its clinical aggressive behaviour.