Cloning, Subcellular Location and Expression Analysis of Grape MYB Gene

Abstract MYB gene plays an important role in plant growth, development and response to abiotic stress. In this study, the Eurasian grape (Vitis vinifera L.) cultivar ‘Yatomi Rosa’ was used as the test material. Two MYB genes, VvMYBB1 gene and VvMYBA3 gene, were obtained by homologous cloning, and their subcellular location, different organs, and expression patterns under stress treatment and hormone induction were obtained. It was found that VvMYBB1 gene and VvMYBA3 gene proteins were located in the nucleus and belonged to nuclear proteins. VvMYBB1 gene and VvMYBA3 gene were highly expressed in roots and flowers. VvMYBB1 gene and VvMYBA3 gene might have a negative effect on the formation of peel color. VvMYB1 gene and VvMYBA3 gene might play an important role in drought resistance and salt stress resistance in grapes. Under the induction of exogenous hormones, the expression of VvMYBB1 gene was higher than that of VvMYBA3 gene under the treatment of IAA, ETH, SA and MeJA. Expression of VvMYBB1 gene was lower than that of VvMYBA3 gene under the treatment of 6-BA, GA3 and ABA. It showed that MYB gene played an important role in the development of different organs of grapes, fruit coloring and response to abiotic stress. This would provide reference for the utilization of grape MYB gene resources.