Effects of Root Restriction Cultivation of Prunus persica on Field Soil Microorganisms and Properties

Root restriction cultivation is a new cultivation method.In this study, we determined the relationships between the microbial composition and properties of the rhizospheric soil after cultivating peach (Prunus persica (L.) Batsch) for three years under root restriction and normal cultivation methods.The dynamic changes in the microbial compositions were analyzed by high throughput sequencing of the V3-V4 regions of bacterial 16S rRNA to explore the responses of rhizosphere bacteria to the different cultivation patterns.The soil properties were determined using experimental methods.The results showed that the abundance of Proteobacteria increased significantly in the soil under root restriction and Bacteroidetes and Actinobacteria were more sensitive than other phyla.The soil organic matter (SOM) content, pH, available nitrogen (AN), available potassium (AK), and available phosphorus (AP) affected rhizospheric microorganisms.In addition, the available nitrogen, available phosphorus, and available potassium concentrations were lower in the root restriction cultivation groups compared with the normal cultivation group.The pH decreased by about 1 unit in the normal control soil after cultivation for 3 years.Our results suggest that the composition of the soil bacteria in the rhizosphere of P. persica responded differently to the twocultivation pattern.The findings obtained in this study may provide insights to facilitate improvements in the soil quality during peach tree cultivation.