Chromatographic fingerprint analysis and quantitative evaluate the rhizomes of Alpinia officinarum Hance (lesser galangal)

The rhizome of Alpinia officinarum Hance is a well-known traditional Chinese medicine (TCM) and has been widely used for the remedy of gastrointestinal diseases. In the present study, a simple and rapid HPLC-DAD was developed for the qualitycontrol of the rhizomes of A. officinarum. Its chemical fingerprint was established using raw material of 15 different origins in China. Similarity analysis (SA) and hierarchical clusting analysis (HCA) were applied to select the qualitative markers. Principal components analysis (PCA) was conducted to select the quantitative markers of the rhizomes of A. officinarum samples from different origins. The constituents were confirmed by (+) electrospray ionization LC-MS. 12constituents were selected as common peaks and 10 of them were confirmed by (+) electrospray ionization LC-MS. Six bioactive constituents including DPHA, galangin flavanone, galangin, galangin 3-methylether, DPHB and DPHC were simultaneous determination by using the HPLC-DAD analysis. The developed method was able to determine the bioactive components with excellent resolution, precision and recovery. The results indicated that chromatographic fingerprint combination with multi-components determination method is suitable for quality assessment of the rhizomes of A. officinarum. 高良姜是著名的中药材, 临床上广泛用于治疗胃肠道疾病。本研究建立了一种简便、快速的HPLC-DAD方法对高良姜进行质量控制。建立基于15个不同产地高良姜样品的指纹图谱, 采用相似性分析(SA)、层次聚类分析(HCA)和主成分分析法(PCA)法对不同产地的高良姜药材及指标成分进行分析, 采用HPLC-ESI-MS法对主要成分进行鉴定。最终确定了12个共有峰, 并对其中的10个主要色谱峰进行了明确的化学指认, 建立了6个活性组分(5R)-5-羟基-1-苯基-7-(4-羟基-3-甲氧基苯基)-3-庚酮(DPHA)、乔松素、高良姜素、高良姜素-3-甲醚、1-苯基-7-(4-羟基-3-甲氧基苯基)-4-烯-3-庚酮(DPHB)和(5R)-5-羟基-1,7-二苯-3-庚酮(DPHC)的同时含量测定方法。该方法具有良好的分离度、精密度和回收率。结果表明, 色谱指纹图谱结合多组分含量测定法是一种适合于高良姜药材质量评价的分析方法。