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2018•Unpublished venueOpen access

RNA extraction from the pennate diatom Asterionella formosa v1

Carine Puppo, Tomas B. Voisin, Brigitte Gontero

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Abstract

Asterionella formosa is a freshwater diatom and as such as a silica cell wall. Therefore classical protocols for RNA extraction do no work. We have settled this protocol that gives high quality RNA (tested by NGS). This protocol was also applied to marine diatoms, Thalassiosira pseudonana and Phaeodactylum tricornutum and gave very good yields.

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What this paper is about

Asterionella formosa is a freshwater diatom and as such as a silica cell wall. Therefore classical protocols for RNA extraction do no work. We have settled this protocol that gives high quality RNA (tested by NGS). This protocol was also applied to marine diatoms, Thalassiosira pseudonana and Phaeodactylum tricornutum and gave very good yields.

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Available abstract

Asterionella formosa is a freshwater diatom and as such as a silica cell wall. Therefore classical protocols for RNA extraction do no work. We have settled this protocol that gives high quality RNA (tested by NGS). This protocol was also applied to marine diatoms, Thalassiosira pseudonana and Phaeodactylum tricornutum and gave very good yields.

Key concepts: Phaeodactylum tricornutum, Thalassiosira pseudonana, Diatom, Extraction (chemistry), RNA, Biology, Botany, Chemistry

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